Molecular pathology. The study of DNA and RNA sequencing, genes, and genetics.
Introduction: In one sense, molecular testing has been used in surgical pathology for several decades in the context of immunohistochemistry, in which antibodies are used to detect and quantify the expression of specific proteins. However, in common parlance, molecular testing refers to testing based on the analysis of nucleic acids such as DNA and RNA. Regardless of whether the disease process is benign or malignant, most genetic testing protocols in surgical pathology are designed to detect somatic or acquired DNA mutations limited to specific cells of the disease process. The recent increase in molecular genetic tests reflects their ability to improve patient care by providing new, independent, or refined information with the potential for clinical stratification of disease subtypes, disease predispositions , prognostic categories, or treatment regimens. While this review will concentrate on the most commonly used molecular techniques in surgical pathology. It is worth not...